High-Precision Molecular Assays for Pharma and Biotech Research
From initial assay development to full qualification, Meadowhawk’s polymerase chain reaction (PCR) lab supports your biodistribution, PK, and PK-PD study with speed, accuracy, and fit-for-purpose requirements. Leveraging other advanced technologies, including cutting-edge LCMS capabilities, our molecular assays deliver a full picture of bioanalytical readouts that are robust and reliable.
Our Molecular Assays and PCR Testing Services deliver data in the shortest time possible.
Talk to a ScientistExpertise: We offer custom end-to-end molecular assay development, from initial design to optimization, tailored to your specific needs.
Collaboration: Building strong partnerships in alignment with your research purposes, we find the right solution with fit-for-purpose assay design and execution to meet your needs.
Comprehensive solutions: Combining PCR with in-house LCMS, ligand binding, and flow cytometry capabilities, we deliver more complete data packages for various modalities in PK and PK/PD studies to advance your program to the next stage.
Precision PCR Power: Advanced Molecular Analysis
At Meadowhawk, our state-of-the-art PCR testing lab offers comprehensive analysis capabilities using conventional PCR, allele specific PCR (AS-PCR), reverse transcriptase PCR (RT-PCR), quantitative PCR (qPCR or real-time PCR), and quantitative reverse transcriptase PCR (RT-qPCR) technologies in both single and multiplex options. These technologies provide highly sensitive detection and quantification of nucleic acids for applications including gene expression profiling, biomarker analysis, and pharmacokinetic analysis of oligonucleotide therapeutics (including their bio-distributions). Our expertise ensures accurate, reliable results to support your most critical research needs.
Accelerate Your Research with Meadowhawk Molecular Assays
Our scientific team provides deep expertise in developing and optimizing fit-for-purpose molecular assays using qPCR and RT-qPCR. We partner with you early in the design phase to tailor tests and studies to your specific research needs.
Our laboratories are equipped with the latest PCR instruments to ensure high-quality data. We use the Applied Biosystems thermocyclers, QuantStudio 7 Pro (with 96-well regular and fast-cycle options), the Invitrogen Qubit 4 Fluorometer, and NanoDrop for precise experimental execution and comprehensive data packages.
A Typical Workflow
Our traditional workflow includes:
- Bead-based homogenization of frozen or preserved tissues (including those in RNA-later).
- Automated magnetic bead-based RNA extraction using Trizol or mirVana kits.
- Quantitation and QC using Qubit, NanoDrop, or RNA gel electrophoresis.
- RT-PCR using either our default two-step method or a custom one-step method (RNA-to-CT, Cell-to-CT).
- Absolute or relative quantification with SYBR-green and Taqman assays.